Patents and papers

Papers

The research results at Kyoto University on the compound natural herbal medicine “KIPPOGEN” have been published in an academic journal.
Paper Summary

Research Paper Name: Extension of lifespan and protection against oxidative stress by an antioxidant herb mixture complex (KPG7) in Caenorhabditis elegans.

Authors:

Takahito Moriwaki, Seiji Kato, Yuichi Kato, Ayaka Hosoki, Qiumei Akiyama*
(* Laboratory for stress response biology, Department of Zoology, Division of Biological Science, Graduate School of Science, Kyoto University)

Publishing Journal:

Journal of Clinical Biochemistry and Nutrition
Article ID: 13-11
Journal of the Society for Free Radical Research JAPAN

Publication Date:

August 8, 2013, Japan


Extension of lifespan and protection against oxidative stress by an antioxidant herb mixture complex (KPG7) in Caenorhabditis elegans.
Key Research Results

Ingestion of KIPPOGEN reduces oxidized protein in the body by approximately 10%.
Effective use of adenosine triphosphate (ATP).
Delay of aging and improvement of survival rates.



The 5th Japanese Society for Regenerative Medicine
(March 8, 2006, Okayama Convention Center)
Published Poster:
“Effect of a Medical Compound with Natural Herbal Medicines as its Main Components on Dementia”

Miyabi Tanaka 1, Taro Shirakawa 2

1 Kippo Science, Ltd. 2 Department of Health Promotion and Human Behavior, Graduate School of Medicine, Kyoto University



The 7th Lecture Texts of the Japan Society for Early Stage of Dementia


Examination of effect of blended herb in dementia

Miyabi Tanaka

Kippo Science, Ltd.

Key Words: Dementia patient, IGF-1, DHEA-S, MMSE, problematic behavior

1.Introduction

Recently, as the result of the increase in life expectancy, dementia and cancer associated with aging have become a large problem for society.

Improvements have been attempted by various methods, and we have tried to address these diseases through oral administration of a medical compound consisting natural herbs as main ingredients. As the result of continuing research, through the blending of a dozen herbs, we have developed a method of improving diseases associated with aging.

People are the healthiest at about the age of twenty, but aging starts at that time. We conducted research to prevent aging based on a hypothesis that disease would disappear if people could be restored to their condition at the age of about twenty.

We focused on two types of hormones, IGF-1 and DHEA. The amounts of hormones secretion begins to decrease around the ages of 25 to 30 in human, and we developed a method of preventing aging, not by externally supplementing the secretion, but rather by increasing the hormones in the body.

IGF-1 mediates the action of growth hormones, and has various effects including growth promotion, insulin-like activity, and cell proliferation.1) Its concentration decreases with age, and it is considered to be important for the evaluation of human growth hormone (HGH) secretion ability. DHEA is called an adrenal androgen, and has drawn attention as a marker that shows biological function from the viewpoint of the control of aging.2)

At this time, we measured the effects of ingestion of the herbal blend on dementia patients, and the changes in the levels of these hormones.


2.Analytical Method
2.1 Subjects
The subjects were one man and one woman randomly selected from among the outpatients or home-care patients of the infirmary of the Natural Substances Medical Research Foundation, which is under the jurisdiction of the Ministry of Health, Labor, and Welfare, or of the contracted medical institute to perform the clinical trial.
2.2 Method

During eight weeks, from December, 2003 to April, 2004, for eight weeks, the trial drug (shown in Table 1) was administered to the subjects, and the observation items of clinical symptoms and side effects were measured on the starting day and eight weeks later to investigate the effect and safety of the trial drug. One packet (3.75 g) of the trial drug was given twice a day. Prior to beginning the trial, items such as the details of the trial and the expected effects and side effects were explained to the subjects, and informed consent for trial participation was obtained.
Trial drug: an herbal blend (product name: KIPPOGEN-R)

2.3 Observation Items
  1. 1. Mini Mental State Examination (MMSE)
  2. 2. Problematic Behavior
  3. 3. Side Effects
    Investigate all events that occurred during the trial period, and treat them as side effects unless it is possible to confidently deny a cause-and-effect relationship between the symptoms and the drug.
  4. 4. Blood Tests (IGF-1, DHEA-S)

3.Results
3.1 Subject A, Female, Age 100

Medical history: She has experienced kidney and heart exacerbations since she was age 70, and going outside became difficult. She became bedridden due to a bone fracture in January of 1999, and dementia and problematic behavior began to be noticeable.

Drug history: No particular concomitant drugs.

3.1.1 Course of Observation Items

Mini Mental State Examination (MMSE): Initial test, 17 points. After eight weeks, 22 points.

・Problematic Behavior

Wandering gradually improved compared to baseline, and wasn’t seen at all after eight weeks. No other problematic behaviors were seen.

・Side Effects

No particular side effects were seen.


Blood Tests (IGF-1, DHEA-S):

IGF-1 DHEA-S
Initial Test 37.6 μg/dl 27ng/ml
After Eight Weeks 42.1 μg/dl 30ng/ml

Table 2. Blood Test Results for Subject A


3.2 Subject B, Male, Age 73

Medical history: Cerebral thrombosis occurred three times between the ages of 46 and 49, and unilateral paralysis and advanced dementia remained. Symptoms such as forgetfulness, wandering, and delusions were found. Until now those symptoms had been gradually progressing.

Drug history: No particular concomitant drugs.

3.2.1 Course of Observation Items

Mini Mental State Examination (MMSE): Initial test, 12 points. After eight weeks, 21 points.

・Problematic Behavior

Wandering gradually improved compared to baseline, and was hardly seen after eight weeks. A gradual improvement was found in day/night reversal, mood swings, violent language and behavior, dirty actions, and resistance to the caregiver compared to baseline.

・Side Effects

No particular side effects were seen.

Blood Tests (IGF-1, DHEA-S):

IGF-1 DHEA-S
Initial Test 176μg/dl 27ng/ml
After Eight Weeks 184μgg/dl 30ng/ml

Table 3. Blood Test Results for Subject B



4.Discussion

We used the trial drug, which was a mixture of natural herbs and designed to activate the body, in dementia patients, and investigated its clinical effects and safety.

In the cognitive function test of the MMSE, the score increased eight weeks after the start of the test and improvement tendency was observed in the subjects. Therefore, we can expect that this trial drug has an improvement effect on dementia in the cognitive function test.

With regard to the study of problematic behavior, for both cases, wandering disappeared after eight weeks. Therefore, we can expect that this trial drug has an effect to improve problematic behavior in dementia.

With regard to the study of the resistance to aging based on blood tests, we measured IGF‑1 (somatomedin C) and DHEA-S (dehydro-epiandrosterone sulfate), which have earned attention as having a close relationship to disease conditions that often occur in the elderly.

IGF-1 level increased in both cases. From this result, clinically significant effects of IGF-1, such as growth promotion, insulin-like activity, and cell proliferation, can be expected. And, a similar effect can be expected in an evaluation of human growth hormone (HGH) secretion.

DHEA-S level also increased in both cases. From this result, it is expected that adrenocortical hormone secretion will increase, for which DHEA-S has clinical significance, and an effect of promoting steroid hormone secretion would be expected to accompany that.

Side effects were not seen in the two cases. Therefore, we believe that there is no particular danger in normal use.

Based on these results, we concluded that this medical compound (herbal blend) consisting natural herbs as main components was found to improve intellectual function and to improve independence in daily activities in dementia patients, and we believe that the trial drug may be useful as an auxiliary treatment for dementia.


5.Conclusion

It has been reported that improvement of conditions in elderly patients receiving care through the use of herbal blends, and improvements in diseases associated with aging, including cancer, Parkinson’s disease, diabetes, hypertension, atherosclerosis, heart disease, rheumatism and collagen disease. This drug also acts to reduce side effects due to pharmaceutical products.

Here, we reported the effectiveness of an herbal blend on dementia, and separately from this test, we performed single photon emission computed tomography (SPECT) imaging and investigated the MMSE scores in dementia patients before and after taking the herbal blend. As a result, the MMSE score increased simultaneously with an increase in blood flow in the brains of dementia patients.

In the future, we will add SPECT to the items observed in this study, further classify the types of dementia, add more clinical cases where food that we present is ingested, and evaluate safety and efficacy.



References

1) Verhelst J, Abs R, Vandeweghe M, Mockel J, Legros JJ, Copinschi G, Mahler C, Velkeniers B, Vanhaelst L, Van Aelst A, De Rijdt D, Stevenaert A, and Beckers A. Two years of replacement therapy in adults with growth hormone deficiency. Clin Endocrinol (Oxf), 1997 Oct, 47(4):485-94.

2)Shealy NC. DHEA: The Youth and Health Hormone. New Canaan: Keats Publishing, Inc, 1996.